Do you know how to buy laboratory supplies? This is a technical job!
The purpose of purchasing laboratory consumables is to ensure that the quality of the reagents and consumables used in the experiment is stable, reliable and economical. Today, Sister Xiaoana chats with her about how to buy commonly used laboratory consumables: centrifuge tubes, pipette tips, test tubes, slides, pipettes, etc. and all reagents (except for special regulations).
First of all, be a qualified laboratory consumable purchaser from the responsibilities
1. The staff responsible for consumables should count all the consumables in the laboratory in time, make a purchase application regularly, and perform acceptance checks when receiving consumables.
2. Laboratory inspectors must strictly abide by the operating procedures, conduct quality inspections on the received kits, and determine the specificity, sensitivity, compliance rate, and lower limit of the reagents for the detection of pathogen nucleic acid.
Start with the two most common types of experimental consumables in the laboratory, and analyze the process that should be followed in the process of purchasing consumables.
Consumables
1. Strictly follow the relevant regulations
The person in charge of the laboratory makes an application plan for purchasing consumables according to the work needs of the room in the middle of each month, combined with the recent consumption of consumables, and provides the consumption and inventory of the previous month, and fills in the requisition form. signature. The compilation and printing are uniformly purchased and managed by the agency.
2. Receipt of consumables
(1) Outer packaging inspection:
The packaging should be intact, undamaged and untainted, and clearly marked-manufacturer name, product name, approval number, production date, expiry date, etc.
(2) Inner packaging inspection:
Whether the inner packaging is damaged or leaking, whether the contents are complete, whether there is a corresponding instruction manual, etc.
(3) The above inspection is completed when the goods arrive, and records are made at the same time:
Classified and stored in the reagent storage area.
3. Quality inspection procedures
Take the detection centrifuge tube as an example
(1) Visual inspection:
Check whether the centrifuge tube is deformed, damaged, or cannot be closed.
(2) Experimental detection:
The centrifuge tube can basically pass the preliminary acceptance after the visual inspection is qualified. If the visual inspection is unqualified or the visual inspection is qualified, there are even frequent bursts, liquid leakage, etc., then the experimenter shall conduct the experimental inspection.
A. Thirty centrifuge tubes were randomly sampled from this batch for experimental testing.
B. After adding half volume of physiological saline to the 30 visually qualified centrifuge tubes, centrifuge them for 20 minutes at 10,000 rpm. If any tube caps are found to burst or liquid leakage occurs, it is deemed that the centrifuge tubes do not meet the requirements. Laboratory experiment requirements, for return processing.
C. After preliminary acceptance, before the batch of centrifuge tubes are put into use, take one, add 50μL of distilled water, and measure it together with the sample to detect whether the batch of centrifuge tubes are contaminated by amplification products.
Take 10 centrifuge tubes and place them in a clean beaker filled with distilled water and soak for 30 minutes to detect whether the batch of centrifuge tubes are contaminated by oil or ester.
D. After no unqualified conditions are found in the above-mentioned tests, the batch of centrifuge tubes can be used.
At the same time, during the experiment, in order to prevent the centrifuge tube from bursting due to heating, a plate was added to the heating module.
(3) If there is any quality problem with the consumables, immediately report to the person in charge of the laboratory in writing, and notify the hospital procurement center to go through the return and replacement procedures with the informed consent of the section director.
4. Storage of consumables
(1) The consumables before aseptic processing are placed in the reagent storage area. According to the daily workload, they are placed in the reagent preparation area, sample preparation area, amplification and product analysis area after regular quantitative processing.
(2) Commonly used consumables are placed in the drawer of the laboratory bench at fixed points and quantitatively according to the daily workload. Special consumables are placed in designated places.
(3) The amount used should be recorded, and the amount used for receiving should be supplemented in time.
(4) Glassware should be placed in a designated location, and be careful to avoid damage.
(5) Before ordering consumables, the quantity should be checked accurately.
Reagent
1. When applying for the purchase of reagents by each department, a written application shall be submitted, and after the department agrees, it shall be submitted to the medical department and unit leaders for approval before the purchase.
2. The reagent name, manufacturer, date of purchase, batch number, expiration date, quantity, etc. should be registered when purchasing reagents. A dedicated person keeps the registration.
3. The storage of reagents should be kept separately according to the different requirements of various reagents, and registration should be made.
4. The quality control of each batch of reagents, including the yin and yang control of the reagent and the yin and yang control of known specimens, shall be recorded.
5. The name, formula, quantity, date, etc. of self-prepared reagents should be registered, and the quality of each batch of reagents should be controlled.
6. Quality control should be carried out regularly or irregularly during the use of reagents. If the reagent is found to be suspicious, stop using it immediately and find the cause.
7. Performance check of the kit:
Perform quality inspection on the performance of the kit, and use a serum plate for testing. Use the kit to be tested to determine each specimen in the serum tray, and use the serum tray as the standard to calculate the sensitivity, specificity and coincidence rate of the tested kit according to the following table and the attached fraction.
(1) A certain number of negative and positive samples and 3 to 5 serially diluted positive samples, the total number of samples is 20.
(2) Perform the experiment according to the instructions provided by the kit. The specificity, sensitivity and coincidence rate of the detection kit; serially diluted specimens can determine the lower limit of the reagent.
Specificity (%)=D/(B+D)*100%
Sensitivity (%)=A/(A+C)*100%
Compliance rate (%)=A+D/(A+B+C+D)*100%
Ideally, the specificity, sensitivity and coincidence rate of the kit are 100%.
(3) If the reagents and consumables have quality problems, immediately report to the person in charge in writing and notify the hospital procurement center in writing to go through the return and replacement procedures.
8. Store the test reagents according to the storage conditions
When every staff member uses or distributes consumables,
It must be observed whether its appearance properties meet the requirements.
The consumables that do not meet the requirements must be reported or returned to the factory.
