Uses and Features of Cell Culture Flasks
The shake flask series culture technology came out in the 1930s. Because of its simplicity and practicality, it was quickly developed into a very important technology in microbial culture and popularized, and was widely used in industrial microbial strain screening and large-scale laboratory fermentation experiments. , seed cultivation, etc. The shake flask series can be divided into high-efficiency shake flask and triangular shake flask.
Cell culture, also known as cell cloning technology, is an important technical means of biological research. The cell shake flask is a special consumable used in the cell culture process. Understanding the characteristics of the cell shake flask and the precautions for use is the premise of cell culture work. Cell shake flasks are generally made of BPA-free PC material or PETG material through injection-stretch-blowing process, and have the following characteristics:
1. The air-permeable membrane area of the 3L and 5L high-efficiency shake flask caps is larger than that of similar products, and is suitable for high-density cell culture. The working volume can be filled to 60-80% of the total volume, and the same volume of shake flask cells yields higher.
2. The arc design of the bottle neck of 3 L high-efficiency shaker is too natural. The size of the bottle neck not only ensures the effective gas exchange space, but also makes it easy to hold and operate with one hand.
3. The 5L high-efficiency shaker bottle is equipped with an ergonomically designed handle, which is convenient for taking the shaker bottle and transferring the liquid.
4. The standard product of high-efficiency shake flask is equipped with 0.2μm sterilization and ventilation. In addition, a liquid transfer cap is optional, which is convenient for aseptic transfer of liquid, and bottle caps suitable for liquid transfer can also be customized according to customer process requirements.
5. The hydrophobic design of the breathable film of the bottle cap will not affect the sealing and ventilation effect of the breathable film after contact with the liquid.
When the cell shaker is used, it needs to be used with a shaker for cell culture. The solvent should not exceed 30%-40% of the shaker. During the culture process, attention should be paid to controlling the rotation speed. Generally, the starting speed is 75-125RPM, which can be adjusted according to the specific situation. . Air-jacketed shakers need to pay attention to temperature control, and water-jacketed shakers need to pay attention to the water level.
