Knowledge of pipette tips and pipettes
1. Appropriate Pipette Tips:
In order to ensure more precision and accuracy, it is recommended that the water absorption rate be within the measurement range of 35%-100% of the suction head.
2. Install the Pipette Tip:
For most brand-name pipettes, especially multichannel pipettes, it is not easy to install the Pipette Tips: in order to pursue a perfect and excellent sealing performance, the tips must be inserted into the pipette sleeve, and the Pipette Tips must be rotated up and down or rocked back and forth. Tighten hard. Some people will use the pipette to frequently hit the tip to tighten, but if this operation will cause the tip to deform and damage the accuracy, it will damage the pipette if it is more serious, so this kind of actual operation should be avoided. Yongyue's multi-type pipettes do not have O-rings, and the Pipette Tips with front stop points are matched with each other. The ideal sealing effect can be achieved with just one squeeze, which is indeed a benefit for users of multi-type pipettes.
3. Dip the tip to the angle and depth:
The operation angle of the tip immersion angle is within 20 degrees of inclination, and it is advisable to keep it vertical; the tip immersion depth is as follows:
The deep part of the tip is immersed in the pipette specification.
2L, 10L1mm
Twenty and one hundred millimeters.
Two hundred liters, one thousand L3-6 mm.
5,000 tons, 10 meters L6-11mm.
4. Tip Rinse:
For normal temperature samples, tip rinsing is beneficial to improve the accuracy, but for high temperature or ultra-low temperature samples, tip rinsing will reduce the accuracy of the actual operation, please pay attention to the user.
5. Absorption speed:
The actual pipetting operation should maintain a stable and appropriate suction rate; if the suction speed is too fast, it is easy for the sample to enter the casing, causing damage to the piston rod and sealing ring, and causing the sample to be polluted by the environment.
