Knowledge of pipette tips and pipettes

How much do you know about pipettes and pipette tips? The working principle of the pipette: the piston realizes the suction and discharge of the liquid through the telescopic movement of the spring. The structure of the pipette: generally includes a control button (different manufacturers have different designs, and this button is usually used to adjust the suction volume), a tip push button, a volume display window, a sleeve, an elastic suction nozzle, and a suction tip. Pipetting mode: With the widespread application of pipetting operations, the range of liquids that can be pipetted continues to expand, and the requirements for precision are getting higher and higher. At present, there are mainly two kinds of pipette technologies to meet the needs of precise pipetting of different liquids: built-in Piston pipetting mode: suitable for routine pipetting operations. The piston is located in the pipette sleeve, there is a section of air isolation between the liquid and the piston, and the piston is not in contact with the liquid. This is a more common pipette in the laboratory. When using it, care should be taken not to contaminate the piston with the sample, otherwise it will cause cross-contamination of the sample. The filter tip is a disposable filter tip.

Because the Filter Pipette Tips are disposable filter tip, the major role of the filter tip during use is to prevent cross-contamination. Unlike other filter types that contain additives that can inhibit enzymatic reactions, the filter tip for biological tissue filtration is composed of Made of pure virgin sintered polyethylene. Hydrophobic polyethylene particles prevent aerosols and liquids from being drawn into the pipette. Cartridge tip filters are machine loaded to ensure that the top is completely unaffected during manufacturing and packaging. They are certified free of RNase, DNase, DNA and pyrogen contamination. Additionally, all filters are radiation pre-sterilized after packaging for enhanced protection of biological samples. The use of filter tips can be used to prevent the pipette from being damaged by the sample, which greatly improves the service life of the pipette. Fuzhou pipette tip with filter element has the advantages of uniform distribution of micropores, high pore density and good air permeability.

Correct operation and precautions of the suction head: Leak detection: The suction head in our hand is called an air displacement suction head. If there is air leakage, our sampling results will definitely not be accurate, which will directly affect our experiments. The final result, the consequences are more serious. So, how to judge whether the suction head is leaking? This requires us to do a simple test on it. First, take a transparent container, fill it with water, put the tip to be tested on the tip, and suck the water. If it is P2, P10, P20, P100, P200 If the liquid level inside the tip drops, please immerse the tip in the liquid level for 1-2 mm, wait for 20 seconds, and observe whether the liquid level inside the tip drops. It is the tip of P1000, P5000, and P10ML. Please hang the tip downward for 20 seconds to observe whether there is liquid dripping. If there is, it means that the tip in your hand is also leaking.

How to ensure the reliability of the pipette in daily use? The feel of pressing the operation button becomes unsmooth, and it feels blocked; the operation button cannot return to the original position by itself after being pressed; after aspiration, the pipette is lifted, and the liquid drips continuously/slowly (even if it absorbs water, This is a normal phenomenon for volatile liquids) After releasing the operation button when aspirating liquid, no liquid is sucked into the tip at all. Do you think the pipette is broken when you encounter a similar situation? It is indeed possible, but more than 90% of this situation is caused by failure to maintain it on time. If the lack of regular maintenance of the pipette will affect the user's use, then the lack of regular calibration will directly affect the reliability of each operation, and such an impact is subtle and not necessarily noticeable. Generally speaking, if there is no problem with the performance of the pipette itself, the accuracy of the pipette will be affected according to the time of use, the frequency of use and the personal habits of the pipette. If it is calibrated regularly, it is guaranteed to perform well between calibrations. Pipette manufacturers recommend that pipettes be calibrated no less frequently than once a year. The tip effectively ensures the accuracy and consistency of the tip size.

The Pipette Tips are truly fully automatic, including motorized Pipette Tips ejection, with unmatched accuracy and repeatability. This tip combines multiple functions in one. At the same time, it saves 30 times the pipetting force than manual pipette tips, which is for light pipetting operations. A pipette tip is a hand-held device commonly found in scientific, biotech, pharmaceutical, and other laboratories for transferring an amount of liquid from one container to another. The suction and discharge ranges of the tips range from the basic dispensing accuracy of graduated glass tubes to the micro-liter incremental dispensing accuracy of complex precision instruments. The Pipette Tips commonly used in life science laboratories everywhere are single-channel air displacement Pipette Tips. Cartridge Pipette Tips are different from other filter types that contain additives that inhibit enzymatic reactions. Electric pipette tips

For long-term continuous use of the tip, the temperature can not exceed 80 °C. 10ml pipette tip supply quotation
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