Laboratory pipette tips Classification and how to choose laboratory pipette tips

As consumables used in conjunction with pipettes, Pipette Tips can generally be divided into: standard tips, Filter Pipette Tips, Low Retention Pipette Tips,Robotic Pipette Tips, and wide-mouth tips according to different applications.

1. Standard tips are the most widely used tips, and common tips can be used for almost all pipetting operations, which is the most economical type of tips.
2. The filter tip is a consumable designed to avoid cross-contamination. The sample taken by the filter tip cannot enter the inside of the pipette, so as to protect the parts of the pipette from contamination and corrosion, which is more important. It can also ensure that there will be no cross-contamination between samples, and is often used in experiments such as molecular biology, cytology, and virology.
3. For experiments with high sensitivity requirements, or precious samples or reagents that are easy to remain, you can choose low-adsorption tips to improve recovery. There are more residues.

The packaging specifications of the tip are mainly bagged and boxed. The boxed ones are sterilized at the factory and can be used directly by the user; the bagged tips are 500 or 1000 per bag, and most users will buy the bagged tips and then put the tips into the tip box. . Boxes are relatively expensive, but bags also increase the risk of contamination during manual loading of tip boxes.

Factors affecting tip quality
The performance indicators of the tip are: low adsorption, filter element, tightness, force of loading and ejecting, no DNase and RNase, no pyrogen, etc.
1. Material
At present, the tips on the market are basically made of polypropylene plastic (colorless and transparent plastic with high chemical inertness and wide temperature range). High-quality tips are generally made of natural polypropylene, while low-cost tips are likely to be recycled polypropylene plastic. Yonyue pipette tip is made of imported polypropylene material that meets the USP Class-VI standard, with high transparency, organic solvent resistance, wide temperature resistance range: -80℃~121℃, no deformation after high temperature and high pressure, high quality raw material belt to high performance.

2. Additives
In addition, most tips will also add a small amount of additives during the manufacturing process. Common ones are: (1) color-developing materials for blue tips and yellow tips; (2) additives to help tips in forming After that, it is quickly separated from the mold. (3) Additives for treating the surface of the low adsorption gun head.
The more additives, the higher the probability of undesired chemical reactions during the pipetting process, but without additives, the production process requirements are relatively high, and few manufacturers can achieve.
Yonyue is supported by the industry's advanced mold design technology. It adopts ultra-fine grinding and molding technology, which can be demolded without release agent, and the inner wall is smooth, which minimizes liquid residues.
3. Biological contamination
More and more users will demand that the tips are RNase-free, DNase-free, pyrogen- and ATP-free, and endotoxin-free. However, high-temperature sterilized tips cannot completely guarantee no biological contamination. To ensure no biological contamination, steps such as gamma ray treatment are also required. Therefore, it is almost impossible for users to process ordinary tips into non-biological contamination tips, and the purchased non-biological pollution-free boxed tips should provide corresponding certificates to indicate the test data of the corresponding items. Yonyue has a 100,000-level dust-free workshop to ensure that the products are free of pyrogen, DNase, RNase and endotoxin.