Welfare is here! Try robotic pipette tips!

"Proper pipetting" sounds like a basic operation that every experimenter has already learned, but in fact:

● After every time the liquid is dispensed, some liquid remains in the tip;
● In the detection experiment, the sample addition operation is standard, but it always contaminates the pictures;
● When processing samples and extracting RNA/DNA, the efficiency is extremely low;
● Obviously using a fully automatic pipetting workstation, but the pipetting is not accurate enough for pictures.

And the "real culprit" that caused all this is often the most overlooked existence - the pipette tip.

You must be thinking: as long as the tip can be installed, it is the tip that can be used. In fact, low-quality tips will not only cause residual contamination to affect the sample, a large amount of liquid will remain, but also cause pipetting errors. Many scientific research Human experiments fail at this step. If the pipette and the tip are not matched accurately, it will affect the sealing and accuracy of the pipetting system. These blood and tears lessons are the precious wealth left by generations of predecessors in the laboratory.

In the use of the pipetting workstation, in addition to the throughput and the application function of the equipment, we also need to pay attention to the adaptability of the equipment to the sample and the accuracy of pipetting, and the easily overlooked tips affect the accuracy of pipetting important part of. Poor matching and poor quality tips will seriously interfere with the normal operation of the experiment, and even contaminate the entire working area, causing unnecessary losses. So how to choose the right consumables? You can refer to the following points when choosing a suction head:


1. According to the experimental needs, select the type and function of the tip

● When the sample volume is unknown or non-uniform, when testing animal or environmental liquid samples (such as blood, urine, swabs, water samples, etc.), black conductive tips are required. The conductive tip can detect when it is inserted into the sample and when it stops, preventing the sample from being inserted too deeply into the sample, which will cause the sample to overflow the container and contaminate the entire process;
● When the sample volume is the same and there is no special requirement for the experiment, just choose a standard tip;
● To avoid cross-contamination or protection, use tips with filter cartridges;
● To prevent the RNA/DNA in the experiment from being degraded and causing erroneous results, you should use tips without RNA/DNase;
● For precious samples with high sensitivity requirements or easy residues, low adsorption tips should be used to improve the recovery rate.


2. Judging the accuracy of the suction head

● Good air-tightness of the suction head can ensure that the instructions issued by the workstation are perfectly executed, which is the key indicator for the selection of the suction head. The air-tightness can be judged by hovering after sucking the liquid;
● The tip should not hang liquid, which will cause inaccurate pipetting. The tip test can be performed by trial.


3. Judging the quality of the suction head

● Tip additives: During the production process of the tip, chemical substances such as color developer and mold release agent will be added. The more additives, the greater the impact on pipetting. Therefore, it is necessary to choose tips with less additives;
● Biological contamination: The tip needs to ensure that the existing biological contamination has been cleaned up, and the tip manufacturer should issue a corresponding inspection report to ensure that there is no impact on the experiment;
● When choosing a conductive tip, special attention should be paid to its conductivity. Only a tip with good conductivity can accurately detect the liquid level and avoid pipetting failure and sample contamination.

If you want to learn more about the characteristics of tips that affect pipetting accuracy? And how to judge the quality and quality of the tip? Come and leave us a message.

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