Talk about the steps of using the cell culture plate

1. Add sample: add 100ul (or 2 drops) of specimen dilution to the coated plate (reserve 2-5 wells for negative and positive control and blank control), dilute the serum to be tested with PBS or saline at 1:20 and then take 10ul and add it to the reaction wells.

2. Add control: add negative control 1-3 wells, positive control 1 well, 100ul of control serum each. Blank control 1 well is vacant.

3. Warming: Shake the reaction plate to mix the sample and then place it in a 37℃ oven or water bath for 20 minutes.

4. Plate washing: dilute 15 ml of concentrated washing solution to 300 ml with distilled water.(1) Hand washing: pour out the contents of the reaction plate wells, fill the wells with washing solution, place them for 30 seconds and then shake them off, repeat this 5 times and then pat dry. (2) Machine wash: 5 times, inject 200ul of washing solution into each well or fill it up, leave it for 30 seconds and then suck it up and pat it dry.

5. Add enzyme working solution: add 100ul (or 2 drops) of enzyme working solution to each well, put the reaction in 37℃ for 20 minutes, then wash the plate 5 times, the operation of washing plate is the same as step 4.

6. Color development and termination: add 50ul (or 1 drop) of each substrate A and B solution to the reaction wells and avoid light for 10 minutes at 37℃. Add 50ul (or 1 drop) of termination solution to each well and mix well to terminate the reaction.

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