What is a cell culture dish and what are the cleaning steps
What is a cell culture dish
A cell culture dish is a laboratory vessel for microbial or cell culture, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. It was originally designed in 1887 by a bacteriologist (Julius Richard Petri, 1852-1921) working under the German biologist Robert Koch, so it is also called "Petri dish". Petri dishes are fragile and brittle, so care and care should be taken when cleaning and handling. It is best to clean the used petri dish in time and store it in a safe and fixed place to prevent damage and breakage.
Generally, it goes through four steps of soaking, brushing, pickling and cleaning.
1. Soaking: New or used glassware should be soaked in water first to soften and dissolve the attachments. Before using new glassware, simply brush it with tap water, and then soak it in 5% hydrochloric acid overnight; used glassware often has a large amount of protein and oil attached, which is not easy to wash off after drying, so it should be immersed in clean water immediately after use for brushing. .
2. Brushing: Put the soaked glassware in the detergent water, and brush repeatedly with a soft brush. Do not leave dead corners and prevent damage to the surface finish of the utensils. Wash and dry the cleaned glassware for pickling.
3. Pickling: Pickling is to soak the above-mentioned utensils in a cleaning solution, also known as acid solution, to remove possible residual substances on the surface of the utensils through the strong oxidation of the acid solution. Pickling should not be less than six hours, usually overnight or longer. Be careful when handling utensils.
4. Rinse: The utensils after brushing and pickling must be fully rinsed with water. Whether the utensils are cleaned after pickling directly affects the success or failure of cell culture. When washing the pickled utensils by hand, each utensil should be "filled with water-empty" repeatedly for at least 15 times, and finally washed with re-distilled water for 2-3 times, dried or dried and then packaged for later use.
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