PCR plate introduction
PCR plate is a kind of polymerase chain reaction (Polymerase Chain Reaction, PCR), which is mainly used as a carrier for primers, Taq DNA polymerase, dNTP, template nucleic acid, Mg, buffer, etc. involved in the amplification reaction.
1. PCR plate material
Its own material is mainly polypropylene (PP) nowadays, which can better adapt to repeated high and low temperature settings in the PCR reaction process, and can achieve high temperature and high pressure sterilization. In order to achieve high-throughput operation in conjunction with a row gun, PCR machine, etc., 96-well or 384-well PCR plates are more commonly used. The plate shape conforms to the SBS international standard, and in order to adapt to the PCR machines of different manufacturers, according to the skirt design, it can be divided into four design modes: no skirt, half skirt, raised skirt and full skirt.
2. Common colors of PCR plates
There are transparent and white, of which white is more suitable for the new type of real-time fluorescent quantitative PCR.
3. Purpose of PCR plate
It is widely used in the fields of genetics, biochemistry, immunity, medicine, etc., not only in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also in the diagnosis of diseases or any place where there is DNA and RNA. It is a one-time and easy-to-use laboratory. Product.
