About 96-well plates for PCR experiments, you will understand in three minutes
The 96-well plate used for PCR experiments needs to pay attention to the problem of biological uniformity when doing the inoculation test. First, inoculate one well to see if the protein concentration is appropriate. If appropriate, connect the following well. However, it should be noted that every time a few holes are connected, the culture bottle should be properly shaken to make it evenly distributed as much as possible before taking it. Don't start from the beginning to the end, the culture bottle will sink if it doesn't move.
Same as ordinary cell culture, please pay attention to the sterility of your cells, culture medium, and utensils when using 96-well plates, especially pay attention to the airflow direction during operation, and do not move objects that may carry bacteria and your hands upwind. Note that high-pressure sterilizers cannot be used.
When using this product for inoculation test, there are two kinds of uniformity of spreading, one is the uniformity between the holes, the other is the uniformity within the holes. In order to achieve uniformity between the holes, it is recommended to use a multi-channel pipette (someone called a row gun) to match the corresponding injection tank. Adding each hole one by one is very difficult to achieve uniformity. There is no secret to the uniformity in the well. The experience is that after adding the cell suspension, do not shake or knock the plate, but directly put it into the incubator to let it adhere to the wall.What are the advantages of 96-well plates for PCR experiments? Come and have a look.
1. It is made of high-quality medical grade polypropylene (PP) material, which is not deformed and has good sealing performance.
2. Operating temperature range: -20℃ to 100℃;
3, high light transmittance and uniform cover design, can be used for real-time fluorescence quantitative experiments (qPCR);
4. Suitable for PCR machines of conventional brands and models; 0.1ml white orifice plate, shorten the optical path, and have higher sensitivity and accuracy.
5. Ultra-thin wall design, rapid and uniform heat conduction; compared with no skirting board, the mechanical strength is higher, and the evaporation and the unevenness of the collected signal caused by the deformation in the reaction are reduced.
6. Molded horizontal and vertical coordinate markings for easy sample identification;
7. The flat surface and hollow bulge design facilitate the adsorption of heat-resistant film and ensure the tightness;
8. The semi-skirt design has a stable structure, and the non-skirt design is easy to cut;
9. Different colors are available; reduce the amount of reagents and save costs.
The 96-well plate for PCR experiments is a disposable consumable in the laboratory. It is widely used in genetics, biochemistry, immunity, medicine and other fields. It is not only used in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also used for disease diagnosis or Any place where there is DNA or RNA.
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