What reagents and consumables are needed for culturing cells?
Cell Culture Basic Consumables
(1) Cell culture vessel:Culture flask: 25, 50, 250ml, etc.Petri Dishes: 10cm, 6cm, etc. Some will use culture flasks or petri dishes, and the corresponding capacity should be selected according to the situation.
(2) Consumables:Pipette Tips, pipettes, Centrifuge Tubes, parafilm, etc.
Cell Culture Basic Reagents
(1) Medium: Generally, it is prepared with incomplete medium + 10% FBS (fetal bovine serum), and sometimes double antibodies are added to prevent cell infection. The specific medium type is selected according to the cell type you are growing.
(2) Trypsin: Short for trypsin, it is used to digest cells. Generally, the digestion time is 1-2 min. If the time is too short, the digestion of the cells will be incomplete. If the time is too long, the cells will be damaged by excessive digestion.
(3) Common reagents: alcohol, PBS buffer.
Cell cryopreservation reagents and consumables
(1) Cryopreservation solution: generally composed of 10% DMSO + FBS + medium, the ratio of FBS and medium depends on the cell type, 90% FBS is generally used for more precious cells, and 50% FBS + 40% for general cells. medium to prepare.
(2) Trypsin and other general reagents for cell culture.
(3) Cryopreservation tubes, centrifuge tubes, centrifuges, pipettes, pipette tips, microscopes, freezing boxes, refrigerators, liquid nitrogen tanks, etc.
In biological cytology research, it is sometimes necessary to culture and analyze cancer cells in vitro. If the cells are to be maintained well, it is necessary to use good serum, such as premium imported fetal bovine serum, its endotoxin is less than 3Eu/ml, which makes the cells healthier and the customer's experiments go more smoothly.
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