Application and classification of petri dishes

A petri dish is a laboratory vessel used for microbial or cell culture, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic, generally used for dry plant material, microbial culture, animal Adherent culture of cells And the operation of laboratory inoculation, streaking, and separation of chrysanthemum.

As a kind of cell culture vessel, petri dish is widely used in biological fields such as bacteria and cell culture. The main structure of an ordinary petri dish includes a dish body and a dish cover, which are integrally formed with a bottom wall and a side wall respectively. Existing petri dishes are basically universal for different culture objects. Universal design often comes at the expense of individualized requirements. When such universal petri dishes are applied to some special culture objects, individualized requirements cannot be fully met, which shows the insufficiency of universal petri dishes.

At present, the cover and bottom of the culture dishes used for bacterial culture are not firmly installed, and the cover is easy to loose,adversely affect normal use. On the other hand, the existing petri dishes have the disadvantage of poor air permeability.
Petri dishes are basically divided into two categories according to the material, mainly plastic and glass, glass can be used for dry plant material, microorganism culture and animal cell adherent culture may also be used. The plastic ones may be polyethylene materials, which are disposable and multiple-use, suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the cultivation of plant materials.

How to choose a petri dish
Petri dishes are divided into flat bottom, U-shaped and V-shaped petri dishes, and petri dishes of different shapes have different uses.
1. To culture cells, usually a flat-bottomed petri dish is used, which is convenient for observation under the microscope, has a clear bottom area, and the height of the cell culture liquid is relatively consistent;
2. A flat-bottomed petri dish should be used to measure the absorbance value, and special attention should be paid to the material;
3. In terms of immunology, when two different lymphocytes are mixed and cultured, they need to be stimulated in contact with each other. At this time, a U-shaped petri dish is generally used, because the cells will gather in a small range due to the effect of gravity;
4. V-shaped petri dishes are often used for cell killing and immunological hemagglutination experiments.

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