Nucleic acid detection is inseparable from the experimental consumables: deep well plate and magnetic tip combs
During the epidemic, nucleic acid detection suddenly changed from a biological term to a well-known hot word. Many people around the world have had more than one personal nucleic acid experience. A key step in nucleic acid detection is nucleic acid extraction, and nucleic acid extraction is inseparable from an important consumable, that is, deep well plates.
Deep-well plates are based on the appearance dimensions of ordinary micro-plates (mainly 96-well plates and 384-well plates), while keeping the length and width in line with SBS international standards, while increasing the depth of the holes to increase the volume of each hole by one. Class laboratory plate. It is especially common in
Uses of deep well plates
After a laboratory obtains a new cell line, the cell line must first be cultured and expanded and then cryopreserved. Cell cryopreservation can firstly provide backup when cells are lost due to contamination and other conditions. In addition, almost all cells undergo a certain degree of variation during the process of culture and passage. In order to maintain the consistency of experimental results, generally cells cannot be cultured for dozens of generations. For reuse, it is necessary to thaw and culture the cryopreserved cells with fewer passages before use.
There are four main points in the cell freezing process: harvesting of cells, use of protective agents, freezing rate and storage environment.
01Storing samples
It can replace the conventional 1.5ml centrifuge tube to store samples, and it can be neatly placed during storage, saving space, and has a large storage capacity, which can withstand -80 ℃ refrigerator. Because of its excellent storage performance, deep well plates are also called storage plates.
02 Processing samples
It can be combined with a discharge gun, high-throughput automatic liquid manipulation instruments and software to achieve high-throughput operations on biological samples, such as protein precipitation, liquid extraction, accounting extraction, etc. Greatly improve the efficiency of sample processing. Can withstand 121 ° high temperature and high pressure sterilization.
03Sampling operation
Commonly used in the automation equipment of various companies, it can be directly placed in the sample chamber of the automatic equipment for injection. Compared with traditional injection, it can not only double the number of samples in the sample chamber, but also realize the realization of samples on 96-well plates. The sample is directly injected after processing, eliminating the tedious work of drawing samples back and forth and placing samples.
04 Application direction
Genomic DNA extraction, plasmid DNA extraction and nucleic acid extraction and purification of various samples. High-throughput automated liquid manipulation, enabling high-throughput manipulations, such as protein precipitation, liquid extraction, animal tissue, bacteria, plants, soil, clinical samples, yeast, etc.
Magnetic tip combs
The magnetic tip combs is mainly used for nucleic acid extraction on the nucleic acid extraction machine with the deep-well plate. In the process of extracting nucleic acid, it can protect the magnetic rod from being polluted and prolong the service life of the magnetic rod.
During the experiment, through the up and down movement of the magnetic rod cover, the mixing, cracking, binding, washing and elution of the sample in the corresponding magnetic bead method reagents are realized; through the coordinated movement of the magnetic rod and the magnetic rod cover, the magnetic beads, The transfer of the magnetic bead-target substance complex; the release of the magnetic bead and the magnetic bead-target substance complex is realized by the separation of the magnetic rod and the magnetic tip combs.
Yongyue Medical® provides deep well plates with various capacity specifications from 120ul to 10ml. The products comply with ANSI standards and are suitable for Single Channel Pipette and automation equipment.
Yongyue Medical® deep well plate has good flatness and can be sealed with sealing film for long-distance transportation. It can be sealed with self-adhesive and silica gel without liquid leakage; at the same time, it is resistant to high temperature (121°C, 20psi, 20min,) and low temperature: (- 80°C), the maximum centrifugal force is more than 3000g.
Yongyue Medical® deep well plate adopts 100,000 grade production and assembly, no DNase/RNase, no pyrogen, no endotoxin, less residual liquid, less wall hanging, and low heavy metal content.
Can be applied to HTS, combinatorial chemistry, library applications (compound storage), immunology (serial dilution), cell culture (yeast growth, bacteria, T cells), DMPK (use with filter plates on manifold), micro Prepared and premixed for next-generation sequencing and more.
