Correct posture for sealing film of PCR plate

Presumably everyone has the experience of "filming", that is, sealing the 96-well PCR plate. Ensure that the sealing film is closely attached to the 96-well plate to prevent the liquid from evaporating, so as to ensure the smoothness of the experiment. Today we will share how to properly seal the film.

Usage and precautions of self-adhesive sealing film: (also applicable to aluminum foil film)

Step 1 Attach the sealing film to the board
Remove a single sheet of sealing film from the ziplock bag and reseal the ziplock bag to maintain an enzyme-free environment. As shown in the picture below, keeping the backing side up, hold the cover film and slowly peel off the backing along the tangent.

Then, stick one end of the adhesive side of the sealing film on the board, and master the distance and angle to avoid subsequent sticking. In the process of pasting, one end is pasted, and the other end is pulled.

Tips

• Be sure to wear gloves
• If using a cover film for single-ended labels, partially remove the backing paper, then anchor the cover film to the board so that it seals over the entire board, and continue to remove the backing paper. This method eliminates curling and rollback of the sealing film
• If using a product with two end labels, peel off the center liner in a continuous smooth motion. Slowly peel off the liner to minimize curling. Be careful not to touch the adhesive surface of the film

Step 2 Lamination
Use a lamination plate to slowly scrape the sealing film so that it all seals to the plate. If there is no special lamination plate, you can find a card with a smooth edge, such as a bank card or a bus card without money.
The lamination step should be performed at least twice horizontally and vertically. Applying enough force (and not too much) is critical to getting a good seal. As shown in Figures A and B below, twice horizontally and twice vertically.

Scrape the lamination plate along all outer edges of the orifice plate at least twice, making sure to apply firm and constant pressure. As shown in Figures C and D above, the holes and edges should be pressed again. For good results, be patient with pictures.
After the sealing film is properly sealed to the board, tear off the joint section along the tangent.

Tips

• When laminating the film, the other hand should stabilize the board to prevent the board from shaking violently

Step 3 Check
After sealing, check the plate carefully to confirm that the film is tightly attached to the plate. Confirm the stick marks around each well, that the entire surface of the plate (including the perimeter) is sealed, and that there is no liquid on the membrane. The sealing film should not be wrinkled, if wrinkles are observed, the plate is not properly sealed.
Tips

• Note that for plates with raised edges, the sealing film may not be positioned correctly on the plate, avoid the film extending up the side walls of the plate
• Leave the sealed plate in place for at least 10 minutes before starting the PCR experiment, the adhesion of the sealing film will increase over time
• If possible, centrifuge with a centrifuge for well plates

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