Treatment of uneven cell distribution during cell culture plate assays
The cell culture plate is made of high quality polystyrene with a round hole and flat bottom, produced using ultra-precise molds and fully automated production processes, and individually packaged in plastic. This cell culture plate is a disposable cell culture plate with tissue culture treatment on the inner surface, which is suitable for the culture of walled cells and also for the culture of suspended cells. The ergonomic design of the cell culture plate makes it easy to operate. The non-slip design on the side makes it easy to hold and reduces the contact area, which can greatly reduce contamination during cell culture.
Some users always gather cells in the peripheral part when conducting cell culture plate test, how to deal with this phenomenon? Is it the quality of the plate?
If it is the latter, and if the plate is shaken in a circle, it is likely that the cells are thrown to the peripheral part due to the centrifugal force, resulting in less cells in the middle and more cells around.
Of course, it is also possible that the quality of the plate or the cell density is too low when spreading the plate. This can be done by putting the plate into the incubator for a few hours of saturation before removing it, and the force should be light when seeding the cells. Remember never to shake with an oscillator, otherwise your cells will be clustered together.
There are also some possible treatments.
1. blowing and beating well before adding.
2. Slowly add from the side or bottom of the multiwell plate.
When you encounter the phenomenon when performing primary culture, some people may think that uneven spreading of glue in the culture dish will cause this phenomenon, because when the mixed vessel segments are added to the culture dish, the vessel segments are evenly distributed in the culture dish under the microscope, and the vessel segments that stick to the wall after 24h are just more around and relatively less in the middle.
