Pipette tip installation and use methods and precautions!

A complete pipetting cycle includes the steps of tip installation - volume setting - aspiration and dispensing - removal of tips. Every step has an operating specification that needs to be followed.

1. Pipette tip installation
For single-channel pipettes, insert the end of the pipette vertically into the tip, and press it slightly to the left and right to tighten it;
For multi-channel pipettes, align the first channel of the pipette with the first tip, insert it obliquely, and shake it back and forth to tighten it;
Do not repeatedly impact the pipette to ensure the airtightness of the tip. Assembling the tip in this way for a long time will cause the parts of the pipette to loosen due to strong impact, and even cause the knob to adjust the scale to get stuck.
2. Capacity setting
When adjusting from a large volume to a small volume, you can turn it counterclockwise to the scale; when adjusting from a small volume to a large volume, you can first adjust the set volume clockwise, and then return to the set volume, which can ensure the best accuracy.
Do not turn the adjustment knob out of the range, otherwise it will damage the mechanism in the pipette.
3. Aspiration and discharge
Press the pipette button to the first position, release the button, and aspirate. Remember not to be too fast, otherwise the liquid will enter the tip too fast and the liquid will be sucked back into the pipette.
Draining liquid Close to the wall of the container, first press to the first gear, pause for a while, and then press to the second gear to drain the remaining liquid.
● Vertical aspiration.
● For 5ml and 10ml pipettes, the tip needs to be immersed in the liquid surface for 5mm, slowly suck the liquid, after reaching the predetermined volume, pause under the liquid surface for 3 seconds, and then leave the liquid surface.
● Slowly release the controller when aspirating, otherwise the liquid will enter the tip too quickly and the liquid will be sucked back into the pipette
● When sucking volatile liquid, it is necessary to rinse the tip 4-6 times to saturate the steam in the sleeve chamber, which can avoid liquid leakage.
4. Correct placement of the pipette
After use, it can be hung upright on the pipette holder, but be careful not to drop it. When there is liquid in the pipette tip, do not place the pipette horizontally or upside down, so as to prevent the liquid from flowing back and corroding the piston spring.
If not in use, adjust the range of the pipette to the maximum scale, so that the spring is in a relaxed state to protect the spring.
5. Common mistakes
1) When assembling the pipette tip, repeatedly hitting the tip will make it difficult to remove the tip and even damage the pipette.
2) When aspirating liquid, the pipette is tilted, resulting in inaccurate pipetting, and the liquid can easily enter the pipette handle.
3) Quickly release the thumb when aspirating, this will force the liquid into a turbulent state and the liquid will rush directly into the inside of the pipette.
4) Press directly to the second position to aspirate (should follow the standard method above).
5) Use a large-capacity pipette to remove small volumes of samples (a pipette with a suitable range should be selected).
6) Lay the pipette with the residual liquid tip flat (the pipette should be hung on the pipette rack).

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