How to choose the right cell culture plate?
The scale of laboratory cell culture is large or small. Generally, bioreactors can be used, culture plates (microplates), culture dishes, culture bottles, etc. can also be used. Nowadays, the cell culture mode using culture plates (microplates) is gaining popularity because it helps to study multiple dynamic variables, reduces experimental time, saves expensive reagents, and improves the safety and efficiency of experimental research Sex, helping 3D and organotypic cell culture and scientific research innovation.
There are many cell culture plates on the market, how to choose the right one?
In today's article, we will introduce the key points of choosing a culture plate. You need to consider the number of holes, the shape of the holes, the color of the microplate, and the surface treatment.
1. Number of holes
Most users choose to conduct tissue culture on culture flasks. However, many applications require growth plates. Because the ideal number depends on the level of throughput you need and whether you have the assistance of robots. It is not impossible to add reagents to 96-well plates completely manually, but it is certainly better to have the help of an electric pipette or robot. Expanding to 384-well plates requires even more automated applications.
2. The shape of the hole
The bottom of the well can be flat bottom, U bottom, V bottom, depending on the cell type and downstream applications. For traditional 2D cell culture (such as HeLa or MDCK cells), especially when imaging or spectrophotometric measurement of the culture is required, F-bottom is usually preferred. For cells without contact inhibition, C-bottom is also good. U-bottom is suitable for suspension culture (such as spheroid cell culture), because the round surface makes it difficult for cells to attach and grow. The V-type (cone bottom) culture plate is mostly used for immunological hemagglutination experiments.
3. The color of the culture plate
The color of the culture plate is also closely related to the application. If you observe the cells with a phase-contrast microscope or with the naked eye, you can choose a transparent culture plate. However, for applications outside the visible light spectrum (such as luminescence or fluorescence), colored perforated plates (such as white or black) are necessary. Cold light samples usually choose a white surface to maximize signal reflectivity, while fluorescent applications greater than 300 nm usually use a black surface to absorb the excitation signal. The colored surface can also prevent signal crosstalk between adjacent holes.
4. Surface treatment
The choice of cell surface treatment depends on whether you are culturing suspension cells or adherent cells. For suspension or spheroid cell culture, it is recommended to use the Yongyue culture plate, which is treated with a patented hydrophobic gel to inhibit cell or protein adhesion. For easily attached adherent cells, a standard tissue culture surface is sufficient.
Yongyue Medical`s culture board products use advanced low-temperature polymer material surface treatment technology and manufacturing processes. The tightness of the combination of the cover and the bottom plate is moderate, which is convenient for ventilation and prevents contamination of the culture plate or evaporation of liquid.
Product Features:
The product types are divided into 6 holes, 12 holes, 24 holes, 48 holes, 96 holes, and 384 holes. Various specifications can satisfy various bacterial cultures;
Packing specification: all single packing:
96 wells are divided into flat bottom, U bottom and V bottom;
U-shaped culture plates are mostly used for culturing suspension cell type cells; V-shaped culture plates are mostly used for immunological hemagglutination experiments;
The product is DNase and RNase free, and pyrogen free.
