Tips for using pipette tips

The performance indicators of pipette tips include: low adsorption, filter element, tightness, force of loading and ejection, no DNase and RNase, no pyrogen pollution, etc. Regarding laboratory pipette tips, you need to know some tips, as follows:
1. Use a suitable pipette tip:
In order to ensure better accuracy and precision, it is recommended that the pipetting volume be within the range of 35%-100% of the tip.
2. Installation of the pipette tip:
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install pipette tips. In order to pursue a good seal, it is necessary to insert the pipette sleeve handle into the tip and turn it left and right or shake it back and forth to tighten it forcefully. And some people will use the pipette to repeatedly hit the tip to tighten. This method is not advisable. Long-term operation will cause the pipette tip to deform and affect the accuracy, and it will also damage the pipette. Such operations should be avoided during the process.
3. The immersion angle and depth of the pipette tip:
The immersion angle of the pipette tip is controlled within 20 degrees of inclination, and it is better to keep it upright; the recommended immersion depth of the pipette tip is as follows:
Pipette specifications & tip immersion depth
2L and 10 L, 1 mm
20L and 100 L, 2-3 mm
200L and 1000 L, 3-6 mm
4. Rinse the pipette tip:
For samples at room temperature, pipette tip rinsing can help improve accuracy; but for high-temperature or low-temperature samples, pipette tip rinsing will reduce the accuracy of the operation. Please pay special attention to users.
5. Liquid suction speed:
The pipetting operation should maintain a smooth and appropriate suction speed; too fast aspiration speed will easily cause the sample to enter the sleeve handle, causing damage to the piston and seal ring and cross-contamination of the sample.

[Suggest]
1. Maintain the correct posture when pipetting; do not hold the pipette tightly all the time, use a pipette with a finger hook to help relieve hand fatigue; you can also change hands frequently.
2. Regularly check the sealing condition of the pipette. Once it is found that the seal is aging or leaks, the sealing ring must be replaced in time.
3. Regularly calibrate the pipette: Choose a pipette tip with good airtightness and reliable quality to avoid inaccurate suction and affect the test results.
4. The order of adding samples is the same each time, especially the order of substrate and stop solution is the same to ensure the same color development time for each well.

Yongyue Medical has a 100,000-level purification workshop to ensure that the products are free of DNase, RNase, and heat source pollution. The products have passed the EU CE certification. Welcome to follow us for more product information!

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