Can pipette tips be autoclaved?

High-pressure steam sterilization is a relatively common method of microbial sterilization, which is suitable for the sterilization of laboratory culture media, food, metal equipment, glass, enamel, dressings and medicines. High temperature and high pressure sterilization can not only kill general bacteria, fungi and other microorganisms, but also kill spores and spores. It is the most reliable and widely used physical sterilization method.
Autoclave sterilization is a kind of moist heat sterilization method. Its sterilization principle is: in a closed steamer, the steam cannot overflow, and the pressure continues to rise, so that the boiling point of the water continues to increase, so that the temperature in the pot also increases.
Under a pressure of 0.1MPa, that is, a steam pressure of 103.4kPa (1.05kg/cm2), the temperature in the pot will reach 121°C and maintain it for 15-30 minutes. When the temperature exceeds the temperature range for optimal physiological activity of cells, cell metabolism slows down, and cell proteins, enzymes and nucleic acids will be permanently destroyed, resulting in irreversible cell death. At this steam temperature, it can quickly kill all kinds of bacteria and their highly heat-resistant spores. It is the most reliable and widely used physical sterilization method.
The advantage of high temperature and high pressure sterilization is that the steam penetration is strong and can kill all microorganisms.
It can be seen that the pipette tip can also be sterilized by high temperature and high pressure. So, what matters should be paid attention to when sterilizing pipette tips? Let's take a look together.
1. Sterilize the tip with newspaper
Put it into the tip box for moist heat sterilization, 121 degrees, 1bar atmospheric pressure, 20 minutes; in order to avoid water vapor trouble, you can wrap the tip box with newspaper, or place it in an incubator after sterilization to dry.
2. When autoclaving, the tip box should be wrapped in newspaper for sterilization
Newspaper wraps can absorb water and can avoid too much water, the most important thing is to prevent re-pollution.
3. Matters needing attention in the sterilization of pipette tips during RNA extraction
Use ordinary EP tubes and pipette tips. Before autoclaving, soak them in DEPC water overnight to remove RNase. After removing DEPC the next day, put them in the pipette tip box for moist heat sterilization. 121 degrees, 15-20 minutes. In order to avoid water vapor troubles, newspapers can be wrapped around the tip box, or placed in an incubator to dry after sterilization. It is best to sterilize directly before each extraction, and do not use long-standing pipette tips to extract RNA.
Advantages of high temperature steam sterilization:
Strong steam heat penetration; high sterilization efficiency; short sterilization time; no chemical or physical pollution during the sterilization process; few control parameters of the sterilization equipment and stable operation; steam sterilization is used to save water and energy. High thermal efficiency.
Yongyue`s pipette tips are made of medical grade polypropylene (PP) material, which meets USP VI grade, has excellent chemical resistance, and can be sterilized at 121 degrees high temperature and high pressure (general electron beam sterilization treatment) .

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