About laboratory pipette tips, you must know
1. Use the appropriate tip:
In order to ensure better accuracy and precision, it is recommended that the pipetting volume be within the range of 35%-100% of the tip.
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the pipette tip: in order to pursue a good seal, you need to insert the pipette sleeve handle into the pipette tip and turn it left and right or shake it back and forth to tighten it forcefully. . There are also people who use the pipette to repeatedly hit the tip to tighten, but this operation will cause the tip to deform and affect the accuracy, and severely damage the pipette, so such operations should be avoided. RAININ's multi-channel pipette does not have an O-ring, and it is matched with a tip with a front stop point. It can achieve an ideal seal with only one light pressure, which is indeed a boon for users of multi-channel pipettes.
The immersion angle of the tip is controlled within 20 degrees of inclination, and it is better to keep it upright; the tip immersion depth is recommended as follows:
Pipette specification tip immersion depth
2L and 10 L 1 mm
20L and 100 L 2-3 mm
200L and 1000 L 3-6 mm
5000 L and 10 mL 6-10 mm
For samples at room temperature, tip rinsing can help improve accuracy; but for samples with high or low temperature, tip rinsing will reduce the accuracy of the operation. Please pay special attention to users.
5. Liquid suction speed:
The pipetting operation should maintain a smooth and appropriate suction speed; too fast aspiration speed will easily cause the sample to enter the sleeve handle, causing damage to the piston and seal ring and cross-contamination of the sample.
[Expert suggestion:]
1. Maintain the correct posture when pipetting; do not hold the pipette tightly all the time, use a pipette with a finger hook to help relieve hand fatigue; change hands frequently if possible.
2. Regularly check the sealing condition of the pipette. Once it is found that the seal is aging or leaks, the sealing ring must be replaced in time.
3. Calibrate the pipette 1-2 times a year (depending on the frequency of use).
4. For most pipettes, before use and after using for a period of time, the piston should be coated with a layer of lubricating oil to maintain the seal; for the RAININ pipettes with regular range, it is also ideal without lubrication. Tightness.
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