About nucleic acid detection reagents, those things that have to be said
The detection principle of nucleic acid detection reagents is based on the unique gene sequence of the virus as the detection target. Through PCR amplification, the target DNA sequence of our choice is increased exponentially. Each amplified DNA sequence can be added to us in advance. A piece of fluorescent-labeled probe is combined to generate a fluorescent signal. The more target genes amplified, the stronger the accumulated fluorescent signal. Therefore, nucleic acid detection is actually to determine whether there is viral nucleic acid in the sample by detecting the accumulation of fluorescent signals.
The relative molecular mass of nucleic acid is very large, and it is widely present in all animal and plant cells and microorganisms. The nucleic acid in the organism often combines with protein to form a nucleoprotein. Different nucleic acids have different chemical composition and nucleotide sequence. Like proteins, nucleic acids are also biological macromolecules. The relative molecular mass of nucleic acid is very large, generally hundreds of thousands to several million.
Nucleic acid carries genetic information and plays an extremely important role in the genetics, mutation and protein biosynthesis of organisms. It is used as genetic material: only in RNA viruses; not as genetic material: it plays a role in the process of DNA control of protein synthesis. mRNA is a direct template for protein synthesis, tRNA can carry specific amino acids, rRNA is a component of ribosomes; catalysis: a kind of enzyme.
Okay, the above is an introduction to the relevant content of nucleic acid detection reagents.
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