In the eyes of most people, scientific research laboratories are full of mystery and high-end atmosphere. In the next period of time, Dian Zijun will take you into the laboratory to learn about the various instruments, consumables and reagents inside, and unveil her mystery. First of all, of course, first introduce the most simple and commonly used instrument-pipette gun.
Pipette gun, also known as pipette, is an instrument commonly used in laboratories for quantitatively pipetting small amounts of liquid.
1. Birth
The birth of the pipette has been less than 100 years. In the early laboratories, when the liquid was quantified, it often relied on the ear-washing ball and the graduated pipette to complete the work. Place the other end of the pipette to inhale, suck the liquid into the pipette, then remove the ear-washing bulb, and quickly block the suction end with your thumb, so that the liquid is placed in the pipette due to pressure , And then according to the required amount of liquid, gently move your thumb to release part of the liquid to the required scale. Such a process is very cumbersome, and many experiments often need to frequently transfer liquid (such as plant DNA extraction), and pipettes also need to be cleaned frequently, so a pipette that can quickly and conveniently transfer liquids came into being.
2. Working principle
The working principle of the pipette gun is actually very simple. It relies on the elastic force of the spring to make the internal piston move up and down, and finally suck or discharge the liquid. Commonly used pipetting guns are often of the air displacement type, that is, when the piston is pressed down, the air in the pipetting gun is pressed out, and the spring is also deformed. After releasing the hand, the spring returns to its original shape, driving the piston to move up, and sucking the external liquid into Inside the tip.
3. Range and quantitative
The pipette gun is used for the transfer of small amounts of liquid, and its scale ranges are 0.5-10μl, 10-100μl, 100-1000μl, 1000-5000μl, etc. There is a scale plate on the pipette gun body, adjust it to the designated scale, you can inhale the corresponding amount of liquid.
It is worth noting that the pipette must be adapted to the corresponding pipette tips, which are usually disposable consumables.
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