Yongyue Medical tells you tips about pipetting tips
1. Appropriate pipette tips:
In order to ensure more precision and accuracy, it is recommended that the water absorption rate be within the measurement range of 35%-100% of the suction head.
2. Install the suction head:
For most brand-name pipettes, especially multi-channel pipettes, it is not easy to install the tip: in order to pursue perfect and excellent sealing performance, the tip must be inserted into the pipette sleeve, rotating up and down or swinging back and forth. Tighten hard. There are also some people who use the pipette to tighten the pipette frequently, but if this operation causes the pipette tip to deform and damage the accuracy, it will damage the pipette if it is more serious, so such actual operation should be avoided. Yongyue`s multi-type pipette does not have an O-ring, and the suction heads with front stop points are matched with each other. The ideal sealing effect can be achieved with only one squeeze, which is indeed a benefit for users of multi-type pipettes.
3. Dip the tip into the angle and depth:
The operating angle of the tip immersion angle is within 20 degrees of inclination, and it is advisable to keep it vertical; the tip immersion depth is as follows:
The deep part of the tip is immersed in the pipette specification model.
2L,10L1mm
Twenty millimeters and one hundred millimeters.
Two hundred liters, one thousand L3-6 mm.
5,000 tons, 10 meters L6-11mm.
4. Suction head rinse:
For samples at room temperature, tip rinsing is helpful to improve accuracy, but for high temperature or ultra-low temperature samples, tip rinsing will reduce the accuracy of the actual operation, please pay attention to it.
5. Liquid suction speed:
The actual pipetting operation should maintain a stable and appropriate liquid absorption rate; too fast aspiration speed can easily cause the sample to enter the casing, causing damage to the piston rod and sealing ring, and causing environmental pollution to the sample.
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